Solid-Phase Edman Degradation. An Automatic Peptide Sequencer
نویسندگان
چکیده
منابع مشابه
Peptide Sequencing by Edman Degradation
By the middle of the twentieth century it was known that proteins were composed of amino acids, but not how these were joined together. Were they arranged in blocks of similar residues? or randomly mixed together? or in repeated patterns? At about that time, methods were devised to provide an answer to these questions: namely, that each individual protein has its constituent amino acid residues...
متن کاملIncreased efficiency in solid-phase Edman degradation of synthetic peptidyl-resins using an oxymethylphenylacetamidomethyl-linkage.
The solid-phase Edman degradation procedure as proposed by Laursen [ 1] is ideally suited to the evaluation of peptidyl-resin products resulting from Merrifield solid-phase synthesis [2,3] of peptides. Incomplete deprotection and incomplete coupling reactions which occur during synthesis have been detected using quantitative Edman degradation [4,5]. However, extended sequencing experiments were...
متن کاملNonradioactive, solid-phase DNase I footprints analyzed on an A.L.F. DNA Sequencer.
Solid-phase DNase I footprinting provides a powerful tool for analyzing the sequence-specific interactions of DNA binding proteins. Classically this type of assay requires radioactively labeled DNA molecules. Substitution of the isotope by fluorescein labeling of the DNA fragments enables the analysis of footprint patterns on a standard automated laser fluorescent (A.L.F.) DNA Sequencer. The co...
متن کاملHigh-throughput sequencing of peptoids and peptide-peptoid hybrids by partial edman degradation and mass spectrometry.
A method for the rapid sequence determination of peptoids [oligo(N-substituted glycines)] and peptide-peptoid hybrids selected from one-bead-one-compound combinatorial libraries has been developed. In this method, beads carrying unique peptoid (or peptide-peptoid) sequences were subjected to multiple cycles of partial Edman degradation (PED) by treatment with a 1:3 (mol/mol) mixture of phenyl i...
متن کاملCombinatorial solid phase peptide synthesis and bioassays.
Solid phase peptide synthesis method, which was introduced by Merrifield in 1963, has spawned the concept of combinatorial chemistry. In this review, we summarize the present technologies of solid phase peptide synthesis (SPPS) that are related to combinatorial chemistry. The conventional methods of peptide library synthesis on polymer support are parallel synthesis, split and mix synthesis and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1971
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1971.tb01366.x